Journal: Molecular Therapy Oncology

Article Title: Engineered Salmonella -mediated c-di-AMP delivery activates STING to remodel the tumor microenvironment

doi: 10.1016/j.omton.2026.201185

Figure Lengend Snippet: Effect of SL disA therapy on T cells and macrophage from tumor samples (A, B) Flow Cytometry analysis of CD3 and CD8 surface markers(A) and CD3 + CD8 + cells statistical graph (B). (C, D) Flow cytometry analysis of CD3 and CD4 surface markers (C) and CD3 + CD4 + cells statistical graph (D). (E, F) Flow cytometry analysis of F4/80 and CD86 surface markers (E) and F4/80 + CD86 + cells statistical graph (F). Data are expressed as mean ± SEM, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, one-way ANOVA with Tukey’s multiple comparisons tests.

Article Snippet: The following antibodies were used: FITC anti-mouse F4/80 (clone CI: A3-1), APC anti-mouse CD86 (clone GL-1), FITC anti-mouse CD3 (clone 17A2), APC anti-mouse CD4 (clone GK1.5), and APC anti-mouse CD8 (clone YTS-169), all purchased from Elabscience Biotechnology Co., Ltd.

Techniques: Flow Cytometry

Journal: bioRxiv

Article Title: Early-life mucosal T cells direct intestinal stem cell fate via a coordinated developmental program

doi: 10.64898/2026.05.08.723752

Figure Lengend Snippet: (A) Co-culture strategy (created with Biorender.com ). Small intestine (SI) was cryopreserved, and stable organoid lines were generated. T cells were isolated from SI tissue (B-E, H-K) or cord blood (F-G) by positive selection. CD3+ cells were co-cultured with established SI organoid lines generated from fetal (B-I) , neonatal (D-E) , or adult (I-K) donors. (B, D, F, H) Representative images from day 6 of co-culture. Scale bar is 530 µm. (C) Data were generated from one organoid and T cell donor per fetal age group and analyzed by one-way ANOVA with Tukey’s post-hoc test. Only significant comparisons are denoted. Data are shown as means ± SEM. (E, G, I) Data were generated from 2-4 organoid and T cell donors each. Data were analyzed via Mann-Whitney and are shown as medians ± IQR. (K) Data were generated from 3 organoid donors and 4 T cell donors and are normalized to the number of organoids generated in mock (no T cell) conditions, represented as fold change. Each dot represents the average of three technical replicate wells (C) or one well of organoids (E, G, I, K) . Data were analyzed via Welch’s t-test and are shown as means ± SEM. Each experiment was repeated 2-3 independent times. Each dot represents one well of organoids. Significance is indicated as follows: * p<0.05; ** p<0.01; *** p<0.001; **** p<0.0001; ns, not significant (p>0.05).

Article Snippet: T cells were isolated via CD3 microbead positive selection (Miltenyi, cat#130-050-101 or 130-097-043) via MS or LS columns (Miltenyi, cat#130-042-201 or 130-042-401) per manufacturer’s instructions (Miltenyi).

Techniques: Co-Culture Assay, Generated, Isolation, Selection, Cell Culture, MANN-WHITNEY